E. coli minimal growth media for beta-galactosidae induction:

A. To 1 liter of distilled H20 add:

1) 6 g. Na₂HPO₄

2) 3 g. KH₂PO₄

3) 0.5 g. NaCl

4) 1 g. NH₄Cl

B. Autoclave above mixture and cool to 50^oC

C. Add with sterile technique to cooled liter of media:

1) 2 ml. 1 M MgSO₄

2) 0.1 ml. 1M CaCl₂

3) appropriate carbon source:

a) for Glucose Minimal Media:
to cooled media add 0.1% sterile glucose
(10 ml sterile filtered 10g/100ml glucose per liter of media)
(1 ml sterile filtered 10g/100ml glucose per 100 ml. of media)

-or-

b) for Lactose + Glucose Minimal Media
to cooled media add 0.1% sterile lactose 0.007% sterile glucose
(10 ml sterile filtered 10g/100ml lactose + 0.7 ml sterile filtered 10g/100ml glucose per liter media)
(1 ml sterile filtered 10g/100ml lactose + 0.07 ml sterile filtered 10g/100ml glucose per 100 ml media)

-or-

c) for Lactose Minimal Media:
to cooled media add 0.1% sterile lactose
(10 ml sterile filtered 10g/100ml lactose per liter media)
(1 ml sterile filtered 10g/100ml lactose per 100 ml media)

Maintained by JG Kunkel

Created: 2/2/97   Modified: 3/1/97