Safety Instructions to Use with Pipet-Aid

• 1 Follow all general laboratory safely regulations.
• 2 Keep this manual easily available to any user.
• 3 Avoid personal injury from chemicals. Wear chemical splash goggles and other appropriate safety equipment and clothing to protect yourself.
• 4 Always work in a way which neither endangers the user nor any other person: Avoid squirting. Only pipette into suitable vessels.
• 5. The Pipet Helper with attached pipette should always be held in a vertical position, tip down.
• 6. Never use force when using this instrument.
• 7. Use the instrument only for pipetting. and within the recommended limits of its chemical resistance and mechanical properties.
• 8. Never use without the recommended membrane filter. The membrane filter is hydrophobic, has a 3 µm pore size, and serves as a barrier to keep out aqueous solutions.
• 9. Take great care so that no liquid enters into the instrument.
• 10. For additional information or help, contact Brinkmann Instruments, Inc.

	Before Pipetting: (1) Compress the Bulb Before attaching the pipette, squeeze the suction bellows.
	(2) Attach the Pipette Hold the pipette as near to its upper end as possible, and carefully insert it into the Pipet Aid adapter until it fits tight. Warning: Never use force. Thin pipettes are particularly liable to break. Avoid the risk of injury! Once the pipette has been securely attached, always hold the instrument in a vertical potion, tip down.
	Pipetting: (3) Fill the pipette - (a) Immerse the pipette tip into the liquid. (b) Press the pipetting lever slowly upwards. Fill the pipette so that the level of liquid is slightly above the required mark.
	Attention: Please take care that no liquid enters into the instrument. This would impair the filtering function and reduce the suction capacity. If liquid does enter the instrument, exchange the filter (see page 10 of manual). Note: The further up the lever is pressed, the stronger the suction effect becomes, and the quicker the meniscus rises.
	In the case of pipettes with a waiting time: As soon as the meniscus in the pipette comes to a standstill, start waiting time as is indicated on the pipette. Wipe the pipette tip a few millimeters upward along the wall of the vessel. In the case of blow-out pipettes: As soon as the meniscus in the pipette tip comes to a standstill, press the small blow-out below to discharge the last drop. Wipe the pipette tip a few millimeters upward along the wall of the vessel.

Note: In the case of large pipettes (> 50 ml) the vacuum contained in the suction bellow does not suffice to draw in all of the liquid at once. Therefore, squeeze the suction bellow again and continue drawing up liquid.

Maintained by JG Kunkel, joe@bio.umass.edu

Last modified: 2/06/97